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Chemical Sciences Seminars

Cancer Metabolomics: Tissues, Body Fluids and NMR

by Ms. Shatakshi Srivastava (Lucknow University)

Thursday, May 9, 2013 from to (Asia/Kolkata)
at Colaba Campus ( AG-80 )
TIFR, Colaba Mumbai 400005
Description NMR metabolomics has been an increasingly active research topic, with recent attempts are being made in gathering unbiased samples of metabolic biomarkers that could serve as snapshots of physiological and pathological states of disease.  A fundamental goal in medical science has become the differentiation of healthy individuals and individuals that have or might develop a disease. It includes an unprejudiced, comprehensive and simultaneous systematic profiling of multiple metabolite concentrations and their cellular and systemic fluctuations in response to toxic insult, genetic manipulation and environmental stress. 

The cancer cells possess a highly unique metabolic phenotype which is characterized by high glucose uptake, increased glycolytic activity, decreased mitochondrial activity, low bio-energetic expenditure and increased phospholipid turnover. In addition to these general alterations in metabolism during malignancy, tissue-specific biochemistry has identified specific endogenous metabolites found in particular tumor types. These metabolic profiles can be readily assessed to monitor the presence and progression of disease. The High resolution NMR spectroscopy in combination with statistical approaches is recently being used to generate a global metabolic profile on patient samples, which can then be used for disease diagnosis.

This talk will explore NMR-based approaches in cancer research for such metabolic profiling in tissue biopsies and body fluids like urine, bile, cerebro-spinal fluid (CSF), serum, tissues extracts and tissues. The aim is to explore the diagnostic ability of NMR spectroscopy towards biomarker identification and pattern recognition in body fluids and tissue specimens obtained from patients suffering with cancer. The research work conducted by me so far, involves extensive application of NMR spectroscopy for:
  1. HRMAS 1H-NMR analysis of tissue biopsies of Squamous Cell Carcinoma (SCC) and benign tumors of oral cavity for differential diagnosis.
  2. 1D- and 2D 1H-13C NMR analysis of urine    of Urinary bladder cancer and normal cases.
  3. Analysis of serum and bile in case of      obstructive jaundice leading to hepato-biliary malignancy by 1H-NMR spectroscopy.
In future, it may become a major diagnostic tool to study different disease-based metabolites as the markers.