Twenty-20 NMR: Rapid Data Acquisition Methods in Protein NMR

With the availability of genomes of several organisms, including that of the human, today, the focus is on to study the expression patterns of all proteins encoded by genes in a particular organism. However, deciphering protein function requires a deeper understanding of the three-dimensional (3D) structure, dynamics and interaction between various proteins. In this direction, multidimensional NMR spectroscopy has provided a powerful arsenal of versatile tools for yielding information on various spectral parameters.  However, all these experiments require long instrument time. One method of overcoming this problem is the so-called joint sampling of two or more indirect dimensions in a higher-dimensional experiment. This approach encodes the information content of higher dimensional spectra onto lower dimensional spectra (2D and 3D) and thus it enables one to acquire the data rapidly. In my presentation, I will first introduce the basic principles of this esoteric spectroscopy with applications to some biological systems.